Plant Health Archives, Volume 1, Issue 5 : 34-36. Doi : 10.54083/PHA/1.2.2023/34-36
Research Article

Published on : 07-Aug-2023

Development of Low Cost Artificial Diet for Mass Production of Entomopathogenic Nematode, Heterorhabditis indica a Strain ICRI EPN-18

  • P. Thiyagarajan
  • Indian Cardamom Research Institute, Spices Board India, Myladumpara, Idukki, Kerala (685 553), India
  • M. Varna
  • Indian Cardamom Research Institute, Spices Board India, Myladumpara, Idukki, Kerala (685 553), India
  • M.A. Ansar Ali
  • Indian Cardamom Research Institute, Spices Board India, Myladumpara, Idukki, Kerala (685 553), India
  • A.B. Rema Shree
  • Indian Cardamom Research Institute, Spices Board India, Myladumpara, Idukki, Kerala (685 553), India

Abstract

Eight different diets were compared for maximum production of entomopathogenic nematode infected Galleria cadavers with cheaper/ low cost in the laboratory. Among eight artificial diet composition tried, low grade milk powder 100 g, low grade glycerin 100 ml, honey 100 ml, corn flour 200 g, wheat bran 100 g, wheat flour 100 g and yeast 50 g produced almost maximum Galleria larvae with EPN infected cadavers and EPN infective juveniles (IJs) with cheaper cost while, the diet containing, ragi powder 100 g instead of milk powder, honey 100 ml, high grade glycerin 100 ml, corn flour 200 g, wheat bran 100 g, wheat flour 100 g and yeast 50 g produced least number of EPN infected Galleria cadavers and EPN IJs. Among the diet compositions, sugar solution 100 ml instead of honey along with other ingredients used, did not emerge or produce larvae from the Galleria eggs.

Keywords

Artificial diet, Elettaria cardamomum, Entomopathogenic nematode, Small cardamom

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